GSM2668643: Und-H4 ChIP; Human gammaherpesvirus 8; ChIP-Seq - SRA

SRX2919003: GSM2668643: Und-H4 ChIP; Human gammaherpesvirus 8; ChIP-Seq
1 ILLUMINA (NextSeq 500) run: 33,398 spots, 2.5M bases, 588,865b downloads

Submitted by: NCBI (GEO)

Study: Identification of symmetric methylation of histone H4 arginine 3 on KSHV genome (H4R3me2s ChIP-Seq)

PRJNA390547 • SRP109223 • All experiments • All runs

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The objective of this study was to identify the symmetric methylation sites on KSHV genome during latency and lytic reactivation Overall design: KSHV infected, TRExBCBL1-RTA cells were induced with doxycycline for 12h to trigger the lytic reactivation, cells were crosslinked and subjected for chromatin isolation and sonication. ChIP assay was performed using anti-H4R3sym antibody, Control IgG antibody, or Histone H4 antibody.

Sample: Und-H4 ChIP

SAMN07237980 • SRS2285006 • All experiments • All runs

Organism: Human gammaherpesvirus 8

Library:

Instrument: NextSeq 500

Strategy: ChIP-Seq

Source: GENOMIC

Selection: ChIP

Layout: SINGLE

Construction protocol: Latent and lytic cells were cross-linked using formaldehyde to fix the protein-DNA for ChIP assay Chromatins were immunoprecipitated using anti-H4R3symmtreic antibody by following standard ChIP protocol

Experiment attributes:

GEO Accession: GSM2668643

Links:

NCBI link: NCBI Entrez (gds)

Runs: 1 run, 33,398 spots, 2.5M bases, 588,865b

Run	# of Spots	# of Bases	Size	Published
SRR5684049	33,398	2.5M	588,865b	2017-06-19

ID:: 4172289

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